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Abbreviations AAV, adeno associated viral; ALP, alkaline phosphatase; BMT, bone marrow transplantation; Cas, CRISPR associated; ChIP, chromatin immunoprecipitation; CRISPR, clustered regularly interspaced short palindromic repeat; CRISPRa, CRISPR activation; CRISPRi, CRISPR interference; dCas9, deactivated Cas9; DE, distal enhancer; Dox, doxycycline; EB, embryoid body; ESC, embryonic stem cell; FBS, fetal bovine serum; GFP, green fluorescent protein; GMEM, Glasgow s Minimum Essential Medium; GWAS, genome wide association studies; ICE, inducible cassette exchange; ICM, inner cell mass, IF, immunofluorescence; KI, knock in; KRAB, KrГјppel associated box; KRAB ZFPs, KRAB containing zinc finger proteins; LIF, leukemia inhibitory factor; LOF, loss of function; MOI, multiplicity of infection; MSC, mesenchymal stem cells; NGS, next generation sequencing; NPC, neural progenitor cell; OCT, optimum cutting temperature; PDLSC, periodontal ligament stem cell; PE, proximal enhancer; RT qPCR, reverse transcription PCR; rtTA, reverse tetracycline transcriptional activator; sgRNA, single guide RNA; SID, SIN3 interacting domain; SNP, single nucleotide polymorphism; TFAM, mitochondrial transcription factor A; TRE, tetracyclin response element; TSS, transcription start site; WT, wild type how to get accutane prescribed to you
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